A COMBINED CELLOBIOSE OXIDASE GLUCOSE-OXIDASE BIOSENSOR FOR HPLC DETERMINATION ONLINE OF GLUCOSE AND SOLUBLE CELLODEXTRINES

被引：40

作者：

NORDLING, M ^{[1
]}

ELMGREN, M ^{[1
]}

STAHLBERG, J ^{[1
]}

PETTERSSON, G ^{[1
]}

LINDQUIST, SE ^{[1
]}

机构：

[1] UNIV UPPSALA,BMC,DEPT BIOCHEM,S-75105 UPPSALA,SWEDEN

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 214卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly specific biosensors can be prepared by immobilizing flavin-containing oxidases in a redox polymer on an electrode surface. By combining one glucose oxidase and one cellobiose oxidase electrode in a flow cell we have made a sensor for flow-injection analysis, or postcolumn quantification, of glucose, cellobiose, and higher cellodextrines in an HPLC system. Samples of different concentrations of glucose and cellobiose, separately or mixed, were injected into the mobile phase and the current response was recorded simultaneously from both electrodes. The recorded response peak heights could be used for calibration curves. The usable measuring ranges were roughly 50 μM-50 mM for glucose and 5 μM-80 mM for cellobiose. Soluble cellodextrines, Glc(1-6), could be separated on a C18 column by isocratic elution and detected by the sensor. © 1993 Academic Press, Inc.

引用

页码：389 / 396

页数：8

共 23 条

[1] CELLOBIOSE OXIDASE, PURIFICATION AND PARTIAL CHARACTERIZATION OF A HEMOPROTEIN FROM SPOROTRICHUM-PULVERULENTUM [J].