CLONING AND CHARACTERIZATION OF THE DROSOPHILA-MELANOGASTER CDK5 HOMOLOG

被引：16

作者：

HELLMICH, MR ^{[1
]}

KENNISON, JA ^{[1
]}

HAMPTON, LL ^{[1
]}

BATTEY, JF ^{[1
]}

机构：

[1] NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892

来源：

FEBS LETTERS | 1994年 / 356卷 / 2-3期

关键词：

CDK5; CDNA; CYCLIN-DEPENDENT KINASE; DEVELOPMENT; DROSOPHILA;

D O I：

10.1016/0014-5793(94)01298-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

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收藏

页码：317 / 321

页数：5

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