CODING STRATEGY OF THE S-RNA SEGMENT OF DUGBE VIRUS (NAIROVIRUS, BUNYAVIRIDAE)

被引:15
作者
WARD, VK [1 ]
MARRIOTT, AC [1 ]
ELGHORR, AA [1 ]
NUTTALL, PA [1 ]
机构
[1] NERC, INST VIROL & ENVIRONM MICROBIOL, MANSFIELD RD, OXFORD OX1 3SR, ENGLAND
关键词
D O I
10.1016/0042-6822(90)90436-U
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The S RNA segment of Dugbe (DUG) virus (Nairovirus; Bunyaviridae) was sequenced from three overlapping cDNA clones and by primer extension. The S RNA is 1712 nucleotides in length and contains one large open reading frame (ORF) of 1326 nucleotides coding for a 49.4-kDa protein on viral complementary (vc) RNA. This protein in size corresponds to the DUG nucleocapsid (N) protein (P. Cash, 1985, J. Gen. Virol. 66, 141-148). The 49.4-kDa product was expressed as a fusion protein with, β-galactosidase in Escherichia coli cells and confirmed as DUG N protein by Western blotting with DUG N-specific monoclonal antibody. An additional ORF of 150 nucleotides coding for a possible 5.9-kDa protein is present in the +1 reading frame, 3′ to the N protein ORF on vcRNA. DUG S segment mRNA was found to be essentially full length. No evidence was obtained for the existence of a smaller mRNA species that could code for a 5.9-kDa protein. Comparisons of the DUG S RNA sequence and predicted N protein amino acid sequence, with the respective sequences of snowshoe hare, La Crosse (bunyaviruses), Punta Toro, Sandfly fever Sicilian (phleboviruses), and Hantaan (hantavirus) viruses, failed to detect any sequence similarity, although the genomic structure of DUG S RNA is similar to that of the S RNA segment of Hantaan (HTN) virus. © 1990.
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页码:518 / 524
页数:7
相关论文
共 26 条
[1]   RAPID EXTRACTION OF HIGH MOLECULAR-WEIGHT RNA FROM CULTURED-CELLS AND GRANULOCYTES FOR NORTHERN ANALYSIS [J].
BIRNBOIM, HC .
NUCLEIC ACIDS RESEARCH, 1988, 16 (04) :1487-1497
[2]   THE COMPLETE SEQUENCE AND CODING CONTENT OF SNOWSHOE HARE BUNYAVIRUS SMALL (S) VIRAL-RNA SPECIES [J].
BISHOP, DHL ;
GOULD, KG ;
AKASHI, H ;
CLERXVANHAASTER, CM .
NUCLEIC ACIDS RESEARCH, 1982, 10 (12) :3703-3713
[3]   MOLECULAR AND BIOCHEMICAL-STUDIES OF THE EVOLUTION, INFECTION AND TRANSMISSION OF INSECT BUNYAVIRUSES [J].
BISHOP, DHL ;
BEATY, BJ .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1988, 321 (1207) :463-483
[4]   AMBISENSE RNA GENOMES OF ARENAVIRUSES AND PHLEBOVIRUSES [J].
BISHOP, DHL .
ADVANCES IN VIRUS RESEARCH, 1986, 31 :1-51
[5]  
Carmichael G G, 1980, Methods Enzymol, V65, P380
[6]   THE NAIROVIRUS GENUS - SEROLOGICAL RELATIONSHIPS [J].
CASALS, J ;
TIGNOR, GH .
INTERVIROLOGY, 1980, 14 (3-4) :144-147
[8]   STRUCTURAL CHARACTERISTICS OF NAIROVIRUSES (GENUS NAIROVIRUS, BUNYAVIRIDAE) [J].
CLERX, JPM ;
CASALS, J ;
BISHOP, DHL .
JOURNAL OF GENERAL VIROLOGY, 1981, 55 (JUL) :165-178
[9]   SMALL VIRAL-RNA SEGMENT OF BUNYA VIRUSES CODES FOR VIRAL NUCLEOCAPSID PROTEIN [J].
GENTSCH, JR ;
BISHOP, DHL .
JOURNAL OF VIROLOGY, 1978, 28 (01) :417-419
[10]   A ONE TUBE REACTION FOR THE SYNTHESIS OF BLUNT-ENDED DOUBLE-STRANDED CDNA [J].
GUBLER, U .
NUCLEIC ACIDS RESEARCH, 1988, 16 (06) :2726-2726