ISOLATION AND CHARACTERIZATION OF THE GENE FOR A YEAST MITOCHONDRIAL IMPORT RECEPTOR

被引:71
作者
MURAKAMI, H
BLOBEL, G
PAIN, D
机构
[1] Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York
关键词
D O I
10.1038/347488a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
WE have previously identified an integral membrane protein (p32) from Saccharomyces cerevisiae as a receptor for protein import into mitochondria, and have localized it to the mitochondrial outer membrane at contact sites1. Here we report isolation of the corresponding mitochondrial import receptor gene, termed MIR1 The deduced amino-acid sequence of p32 shows roughly 40% identity with proteins of bovine heart2 and rat liver3 that have been suggested to be mitochondrial phosphate carriers. Haploid cells carrying a disrupted MIR1 allele were unable to grow on a non-fermentable carbon source but grew in media containing glucose, indicating that the MIR1 protein is essential for mitochondrial function. Compared with wild type, amounts of some mitochondrial proteins were markedly reduced in cells containing a disrupted MIR1 allele, whereas levels of others were unchanged. This indicates that yeast contains more than one pathway for protein import into mitochondria. © 1990 Nature Publishing Group.
引用
收藏
页码:488 / 491
页数:4
相关论文
共 24 条
[1]  
BRANDRISS MC, 1988, PROTEINN TRANSFER OR, P655
[2]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[3]  
FEINBERG B, 1988, YEAST PRACTICAL APPR, P147
[4]  
FERREIRA GC, 1989, J BIOL CHEM, V264, P15628
[5]   PRE-MESSENGER-RNA SPLICING [J].
GREEN, MR .
ANNUAL REVIEW OF GENETICS, 1986, 20 :671-708
[6]   COMPLIATION AND COMPARISON OF THE SEQUENCE CONTEXT AROUND THE AUG STARTCODONS IN SACCHAROMYCES-CEREVISIAE MESSENGER-RNAS [J].
HAMILTON, R ;
WATANABE, CK ;
DEBOER, HA .
NUCLEIC ACIDS RESEARCH, 1987, 15 (08) :3581-3593
[7]   UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].
HENIKOFF, S .
GENE, 1984, 28 (03) :351-359
[8]  
KAPLAN RS, 1989, METHOD ENZYMOL, V173, P732
[9]   A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].
KYTE, J ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132
[10]  
LAWSON JE, 1988, J BIOL CHEM, V263, P14812