2 NATURALLY-OCCURRING ISOFORMS AND THEIR EXPRESSION OF A GLUCOCORTICOID RECEPTOR GENE FROM AN ANDROGEN-DEPENDENT MOUSE-TUMOR

被引：20

作者：

KASAI, Y

机构：

[1] Department of Pharmacology, Kawasaki Medical School, Kurashiki City, 701-01

来源：

FEBS LETTERS | 1990年 / 274卷 / 1-2期

关键词：

D O I：

10.1016/0014-5793(90)81339-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated cDNAs encoding the glucocorticoid receptor from an androgen-dependent mouse tumor, Shionogi Carcinoma 115. The nucleotide sequence of the receptor revealed two different forms, designated as SC-GR I and SC-GR II. Both forms have a one-base substitution in the DNA binding domain of the wild-type mouse glucocorticoid receptor. Furthermore, SC-GR II has a three-base insertion in the interfinger region of this domain. By expressing the receptor cDNAs in cultured cells with MMTV-CAT reporter plasmid, SC-GR II was found to have about half of the activity of the wild-type mouse glucocorticoid receptor induced by either physiological or pharmacological doses of dexamethasone. © 1990.

引用

页码：99 / 102

页数：4

共 31 条

[1] NEGATIVE REGULATION BY GLUCOCORTICOIDS THROUGH INTERFERENCE WITH A CAMP RESPONSIVE ENHANCER [J].