ELECTROCHEMICAL STUDIES ON TETRATHIAFULVALENE-TETRACYANOQUINODIMETHANE MODIFIED ACETYLCHOLINE CHOLINE SENSOR

被引：33

作者：

PANDEY, PC ^{[1
]}

TRANMINH, C ^{[1
]}

LANTREIBECQ, F ^{[1
]}

机构：

[1] BANARAS HINDU UNIV, DEPT CHEM, VARANASI 221005, UTTAR PRADESH, INDIA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 31卷 / 02期

关键词：

D O I：

10.1007/BF02921785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensor for acetylcholine/choline is described using a tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) electrode modified with acetylcholine esterase (AChE) and choline oxidase (ChO) enzymes. DC cyclic voltammetry and impedance measurements of the enzyme-modified TTF-TCNQ electrode that indicate the regeneration of choline oxidase at the electrode surface are reported. Effective electrochemical rate constants for the present enzyme electrode are calculated using the expressions derived by Albery et al. (1), which show the enzyme kinetics as the rate-limiting step. The values of the effective electrochemical rate constants are close to those reported by Hale and Wightman (2). The application of the sensor is described for the determination of fluorode ion and nicotine based on the reversible inhibition of AChE activity. The range of detection of fluoride ion and nicotine is found to be 5 X 10(-6) to 5 X 10(-4)M.

引用

页码：145 / 158

页数：14

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