STUDIES OF A NOVEL MEMBRANE FOR AFFINITY SEPARATIONS .1. FUNCTIONALIZATION AND PROTEIN COUPLING

被引：47

作者：

BAMFORD, CH ^{[1
]}

ALLAMEE, KG ^{[1
]}

PURBRICK, MD ^{[1
]}

WEAR, TJ ^{[1
]}

机构：

[1] KODAK LTD, HARROW HA1 4TY, MIDDX, ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 606卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85253-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A study is presented of the potentialities of an electrostatically-spun poly(ether-urethane-urea) as an affinity separation membrane. The spinning process produces a fibrous network with a high internal surface area. A variety of chemical methods has been used for functionalising and activating the membrane. Assessments of the capacities of the activated membranes for covalent coupling of protein A and human immunoglobulin G have given very encouraging data. Non-specific adsorption of both proteins by the inactivated polymer was negligible. Geometric considerations suggest that the limiting factor determining protein coupling is the accessible surface area rather than the number of coupling sites.

引用

页码：19 / 31

页数：13

共 14 条

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