REDUCTION OF NITRIC-OXIDE PRODUCING ACTIVITY ASSOCIATED WITH IN-VITRO AGING IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELL

被引：113

作者：

SATO, I

MORITA, I

KAJI, K

IKEDA, M

NAGAO, M

MUROTA, S

机构：

[1] TOKYO MED & DENT UNIV, GRAD SCH,DEPT PHYSIOL CHEM,1-5-45 YUSHIMA, BUNKYO KU, TOKYO 113, JAPAN

[2] TOKYO MED & DENT UNIV, FAC DENT, DEPT GERIATR MED, BUNKYO KU, TOKYO 113, JAPAN

[3] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL, DEPT BIOCHEM, ITABASHI KU, TOKYO 173, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the involvement of nitric oxide (NO) in the pathogenesis of the cardiovascular system with aging, we studied the basal release of NO in cultured endothelial cells isolated from normal human umbilical veins (HUVECs) at different population doubling levels (PDLs). The release of NO was assayed by a newly established, highly sensitive assay system monitoring guanosine 3':5'-cyclic monophosphate (cGMP) formation in a pig kidney epithelial cell line cocultured with HUVECs. The cGMP formation by NO released from HUVECs decreased 3.3-fold between PDLs 8 (14.1 pmol/ml) and 50 (4.3 pmol/ml) (P < 0.01). The result suggests that the vaso-constrictive activity of endothelial cells increases during in vitro aging which may reflect the increased vascular disorders in aged people. © 1993 Academic Press, Inc.

引用

页码：1070 / 1076

页数：7

共 28 条

[1] RELEASE OF ENDOTHELIN FROM THE PORCINE AORTA - INHIBITION BY ENDOTHELIUM-DERIVED NITRIC-OXIDE [J].