A NOVEL IMMUNOASSAY OF SMOOTH-MUSCLE MYOSIN HEAVY-CHAIN IN SERUM

被引：39

作者：

KATOH, H

SUZUKI, T

YOKOMORI, K

SUZUKI, S

OHTAKI, E

WATANABE, M

YAZAKI, Y

NAGAI, R

机构：

[1] UNIV TOKYO,DEPT INTERNAL MED 3,TOKYO 113,JAPAN

[2] UNIV TOKYO,DEPT PEDIAT SURG,TOKYO 113,JAPAN

[3] SAKAKIBARA MEM HOSP,CARDIAC UNIT,SHINJUKU KU,TOKYO,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 185卷 / 01期

关键词：

SMOOTH MUSCLE MYOSIN HEAVY CHAIN; AORTIC DISSECTION; MONOCLONAL ANTIBODY; ELISA; SANDWICH;

D O I：

10.1016/0022-1759(95)00104-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a double monoclonal sandwich enzyme immunoassay to measure smooth muscle myosin heavy chain (MHC). Analytical performance of the assay showed reliable detection of smooth muscle MHC in human sera. The mean of the smooth muscle MHC level in normal human sera was 0.9 +/- 0.9 ng/ml. In sera of patients with aortic dissection, the smooth muscle MHC level sharply elevated at the onset and rapidly decreased to normal levels. Immunoassay of smooth muscle MHC in serum is a promising method for biochemical diagnosis of smooth muscle disorders.

引用

页码：57 / 63

页数：7

共 18 条

[1] PROGRESS IN THE DIAGNOSIS AND MANAGEMENT OF AORTIC DISSECTION [J].