STABILITY OF BLOOD (PRO)VITAMINS DURING 4 YEARS OF STORAGE AT -20-DEGREES-C - CONSEQUENCES FOR EPIDEMIOLOGIC RESEARCH

被引：104

作者：

OCKE, MC

SCHRIJVER, J

OBERMANNDEBOER, GL

BLOEMBERG, BPM

HAENEN, GRMM

KROMHOUT, D

机构：

[1] TNO NUTR,DEPT EXPTL BIOL,ZEIST,NETHERLANDS

[2] NATL INST PUBL HLTH & ENVIRONM PROTECT,DIV PUBL HLTH RES,3720 BA BILTHOVEN,NETHERLANDS

来源：

JOURNAL OF CLINICAL EPIDEMIOLOGY | 1995年 / 48卷 / 08期

关键词：

ATTENUATION; FROZEN STORAGE; PLASMA; STABILITY; VITAMINS; WHOLE BLOOD;

D O I：

10.1016/0895-4356(94)00232-F

中图分类号：

R19 [保健组织与事业（卫生事业管理）];

学科分类号：

摘要：

We studied the effects of frozen storage on (pro)vitamin concentrations in EDTA-plasma and whole blood. Aliquots from 55 samples were analyzed before storage and after 3, 6, 12, 24, 36 and 48 months at -20 degrees C. Dramatic decreases occurred for EDTA-plasma concentrations of vitamin E between 6 and 12 months, vitamin A, total carotenoids and beta-carotene after 1 year, and whole blood niacin. A smaller decrease was observed for folic acid at 1 year of storage, but the level remained constant thereafter. The vitamins D, B-6, B-12 (EDTA-plasma), B-1 and B-2 (whole blood) showed no decline during 4 years of storage. With the exception of folic acid, the observed decreases varied considerably among subjects. Therefore using EDTA-plasma stored longer than 1 year at -20 degrees C will result in highly attenuated odds ratios when assessing the relationship between vitamin A, carotenoids, or vitamin E with a given disease. Attenuation will also occur when using niacin concentrations in whole blood stored for 4 years at -20 degrees C.

引用

页码：1077 / 1085

页数：9

共 34 条

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