The mono- and oligonucleotides in total pancreatic ribonuclease digests of purified Escherichia coli B alanine transfer ribonucleic acid peak 1 were separated on DEAE-cellulose columns in 7 M urea. Certain oligonucleotides were further separated by paper chromatography or by DEAE-Sephadex A-25 column chroma tography. Their sequences and the properties of a new minor base were studied by alkaline hydrolysis and bydigestion with ribonuclease T1-takadiastase, snake venom diesterase, or micrococcal nuclease. © 1969, American Chemical Society. All rights reserved.