CHEMICAL STUDIES ON AMINO ACID ACCEPTOR RIBONUCLEIC ACIDS .8. DEGRADATION OF PURIFIED ALANINE ESCHERICHIA COLI B TRANSFER RIBONUCLEIC ACID BY PANCREATIC RIBONUCLEASE

被引:11
作者
ALVINO, CG
REMINGTON, L
INGRAM, VM
机构
[1] Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts
关键词
D O I
10.1021/bi00829a040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mono- and oligonucleotides in total pancreatic ribonuclease digests of purified Escherichia coli B alanine transfer ribonucleic acid peak 1 were separated on DEAE-cellulose columns in 7 M urea. Certain oligonucleotides were further separated by paper chromatography or by DEAE-Sephadex A-25 column chroma tography. Their sequences and the properties of a new minor base were studied by alkaline hydrolysis and bydigestion with ribonuclease T1-takadiastase, snake venom diesterase, or micrococcal nuclease. © 1969, American Chemical Society. All rights reserved.
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页码:282 / +
页数:1
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