ENZYMATIC-ACTIVITIES OF P-450(11-BETA)S EXPRESSED BY 2 CDNAS IN COS-7 CELLS

被引：65

作者：

MOROHASHI, K

NONAKA, Y

KIRITA, S

HATANO, O

TAKAKUSU, A

OKAMOTO, M

OMURA, T

机构：

[1] OSAKA UNIV, SCH MED, DEPT BIOCHEM, KITA KU, OSAKA 530, JAPAN

[2] NARA MED UNIV, DEPT ANAT, KASHIHARA, NARA 634, JAPAN

[3] OSAKA UNIV, SCH MED, DEPT MOLEC & PHYSIOL CHEM, KITA KU, OSAKA 530, JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 107卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression plasmids were constructed using two cDNA clones of P-450(llβ), pcP-450-(llβ)-2, and pcP-450(llβ)-3 (Morohashi et al. (1987) J. Biochenu 102, 559-568 and Kirita et al. (1988) J. Biochem. 104, 683-686), and introduced into COS-7 cells by electroporation. The expression of P-450(llβ) proteins and their localization in the mitochondria were demonstrated by immunoblotting, immunofluorescence microscopy, and immunoelectron microscopy. The enzymatic activities of the expressed P-450(llβ)s were determined using deoxycorticosterone (DOC), deoxycortisol, and corticosterone as substrates. Though the activities of the two P-450(llβ)s for 11-, 18-, and 19-hydroxylation of DOC were almost equal, the production of 18-hydroxycorticosterone and aldosterone from corticosterone by P-450(llβ)-3 was greater than that by P-450(llβ)-2. © 1990 COPYRIGHT, 1990 BY THE JOURNAL OF BIOCHEMISTRY.

引用

页码：635 / 640

页数：6

共 27 条

[1] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].