GALANIN EVOKES A CYTOSOLIC CALCIUM BURSTING MODE AND HORMONE-RELEASE IN GH3/B6 PITUITARY-CELLS

被引：20

作者：

GUERINEAU, N ^{[1
]}

DROUHAULT, R ^{[1
]}

CORCUFF, JB ^{[1
]}

VACHER, AM ^{[1
]}

VILAYLECK, N ^{[1
]}

MOLLARD, P ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,NEUROPHYSIOL LAB,CNRS,URA 1200,146 RUE LEO SAIGNAT,F-33076 BORDEAUX,FRANCE

来源：

FEBS LETTERS | 1990年 / 276卷 / 1-2期

关键词：

GALANIN; CALCIUM; PROLACTIN; GROWTH HORMONE; GH3; CELL;

D O I：

10.1016/0014-5793(90)80520-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of galanin on secretion and cytosolic free Ca2+ concentration ([Ca2+]i) have been studied in GH3/B6 pituitary cells. Prolactin (PRL) and growth hormone (GH) release was measured in column perifusion experiments; [Ca2+]i was monitored in single cells by dual emission microspectrofluorimetry using indo-1 as intracellular Ca2+ probe. Galanin (0.1-1 nM) caused PRL and GH release coincident with a modest rise in [Ca2+]i. The increase in [Ca2+]i comprises the establishment of characteristic long-lasting bursts of [Ca2+]i transients. Galanin acts on Ca2+ entry through voltage-gated Ca2+ channels since there was no response to the peptide when Cd2+ - a Ca2+ channel blocker - was added to the bath solution. The stimulation of bursting activity by galanin may provide a fine Ca2+-signalling mechanism which maximally stimulates hormone release while avoiding refractory periods.

引用

页码：111 / 114

页数：4

共 21 条

[1] GALANIN RECEPTORS IN A HAMSTER PANCREATIC BETA-CELL TUMOR - IDENTIFICATION AND MOLECULAR CHARACTERIZATION [J].