STEM-LOOP STRUCTURES AT THE 3'-END OF TOBACCO RUBISCO LARGE SUBUNIT MESSENGER-RNA

被引:5
作者
AKADA, S
XU, YQ
MACHII, H
KUNG, SD
机构
[1] UNIV MARYLAND,CTR AGR BIOTECHNOL,MARYLAND BIOTECHNOL INST,1206 HJ PATTERSON HALL,COLLEGE PK,MD 20742
[2] UNIV MARYLAND,DEPT BOT,COLLEGE PK,MD 20742
关键词
chloroplast rbcL; expression in E. coli; inverted repeat sequence; mRNA stability; Recombinant DNA; transcriptional terminator;
D O I
10.1016/0378-1119(90)90387-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The are two inverted repeat nucleotide (nt) sequences, each capable of forming a stem-loop structure (sls) at the 3′ end of the tobacco Rubisco large subunit mRNA (rbcL). The smaller sls is followed by a larger sls. The in vivo functions of the 3′ sls of the rbcL mRNA were characterized using the Escherichia coli system. S1 mapping of the rbcL transcripts synthesized in E. coli revealed that the 3′ end of a major transcript in the bacterial cell is almost identical to the 3′ end of authentic chloroplast (cp) rbcL mRNA. This native 3′ end is located 4 nt downstream from the larger sls for the cp mRNA and 6 nt for the bacterial transcript, respectively. Deletion expriements show that the larger sls is essential for producing the native 3′ end of rbcL mRNA in E. coli. The sls do not function an an efficient transcription terminator but can stabilize upstream mRNA segments in vivo. © 1990.
引用
收藏
页码:195 / 199
页数:5
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