A TANDEM REPEAT OF THE SPKK PEPTIDE MOTIF INDUCES PSI-TYPE DNA STRUCTURES AT ALTERNATING AT SEQUENCES

被引：12

作者：

BAILLY, F

BAILLY, C

COLSON, P

HOUSSIER, C

HENICHART, JP

机构：

[1] UNIV LIEGE,CHIM MACROMOLEC & CHIM PHYS LAB,B-4000 LIEGE,BELGIUM

[2] UCB PHARMACEUT,B-1420 BRAINE LALLEUD,BELGIUM

来源：

FEBS LETTERS | 1993年 / 324卷 / 02期

关键词：

DNA CONDENSATION; HISTONE; CIRCULAR DICHROISM; DNA SEQUENCE-SPECIFICITY; BASIC PEPTIDE; TRANSCRIPTION;

D O I：

10.1016/0014-5793(93)81389-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between a tandem repeat of the SPKK peptide motif and calf thymus DNA or several polynucleotides has been investigated by circular dichroism. The octapeptide SPKKSPKK does not induce any important changes in the CD spectra of the polynucleotides poly(dG) . poly(dC), poly(dG-dC) . poly(dG-dC) and poly(dA) . poly(dT) while the spectrum of calf thymus DNA is slightly modified. Binding of this basic peptide to the alternating copolymer poly(dA-dT) . poly(dA-dT) results in a marked PSI-type condensation in a manner similar to that induced by the entire C-terminal domain of histone H1.

引用

页码：181 / 184

页数：4

共 31 条

[1] ROLES OF H-1 DOMAINS IN DETERMINING HIGHER-ORDER CHROMATIN STRUCTURE AND H-1 LOCATION [J].