N-TERMINAL FRAGMENTS OF INTESTINAL CHOLECYSTOKININ - EVIDENCE FOR RELEASE OF CCK-8 BY CLEAVAGE ON THE CARBOXYL SIDE OF ARG(74) OF PROCCK

被引:8
作者
BLANKE, SE
JOHNSEN, AH
REHFELD, JF
机构
[1] Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen
关键词
POSTTRANSLATIONAL PROCESSING; ENDOPROTEOLYSIS; IMMUNOAFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY;
D O I
10.1016/0167-0115(93)90259-B
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
From porcine duodenal mucosa we have identified three major procholecystokinin (proCCK) fragments: desoctaCCK-33, desnonaCCK-33 and desnonaCCK-39. (DesoctaCCK-33 means CCK-33 devoid of the 8 C-terminal amino acids, etc.) The fragments were purified by immunoaffinity chromatography and three steps of reverse phase HPLC monitored by a radioimmunoassay specific for the N-terminal part of CCK-33. The structures could be deduced from the proCCK sequence by N-terminal sequence determination and mass spectrometry. Whereas desnona-fragments of CCK have been described before, this is the first finding of a desoctaCCK, and it indicates that CCK-8 is released from the longer forms by endogenous cleavage of the Arg-Asp-bond. A carboxypeptidase B-like exopeptidase subsequently must produce the desnona-fragments by removing the arginine residue.
引用
收藏
页码:575 / 582
页数:8
相关论文
共 18 条