CLONING OF CDNA-ENCODING RAT PHOSPHOLIPASE C-BETA-4, A NEW MEMBER OF THE PHOSPHOLIPASE-C

被引：25

作者：

KIM, MJ ^{[1
]}

BAHK, YY ^{[1
]}

MIN, DS ^{[1
]}

LEE, SJ ^{[1
]}

RYU, SH ^{[1
]}

SUH, PG ^{[1
]}

机构：

[1] POHANG UNIV SCI & TECHNOL,DEPT LIFE SCI,POB 125,POHANG 790600,SOUTH KOREA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase C-β4(PLC-β4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-β4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-β1, human PLC-β2, and rat PLC-β3, respectively. The amino acid sequence of PLC-β4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-β subtypes, suggesting that the PLC-β4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila. © 1993 Academic Press, Inc.

引用

页码：706 / 712

页数：7

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