NEW VECTORS FOR DIRECT CLONING OF PCR PRODUCTS

被引：37

作者：

CHA, J

BISHAI, W

CHANDRASEGARAN, S

机构：

[1] JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT ENVIRONM HLTH SCI,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,DEPT MED,DIV INFECT DIS,BALTIMORE,MD 21205

来源：

GENE | 1993年 / 136卷 / 1-2期

关键词：

RECOMBINANT DNA; T/A CLONING VECTORS; PHAGE M13MP;

D O I：

10.1016/0378-1119(93)90498-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

引用

页码：369 / 370

页数：2

共 6 条

[1] NOVEL NON-TEMPLATED NUCLEOTIDE ADDITION-REACTIONS CATALYZED BY PROCARYOTIC AND EUKARYOTIC DNA-POLYMERASES
CLARK, JM
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (20) : 9677 - 9686
[2] CONSTRUCTION OF NEW T-VECTORS FOR DIRECT CLONING OF PCR PRODUCTS
ICHIHARA, Y
KUROSAWA, Y
[J]. GENE, 1993, 130 (01) : 153 - 154
[3] CONSTRUCTION OF T-VECTORS, A RAPID AND GENERAL SYSTEM FOR DIRECT CLONING OF UNMODIFIED PCR PRODUCTS
MARCHUK, D
DRUMM, M
SAULINO, A
COLLINS, FS
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (05) : 1154 - 1154
[4] MESSING J, 1983, METHOD ENZYMOL, V101, P20
[5] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
NICKLEN, S
COULSON, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5463 - 5467
[6] EFFICIENT CLONING OF FRAGMENTS OF THE POLYMERASE CHAIN-REACTION DIRECTLY INTO THE SINGLE STRANDED BACTERIOPHAGE M13MP18
ZIMMER, W
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (03) : 773 - 774

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