ATP BINDING TO BOVINE SERUM-ALBUMIN

被引：22

作者：

BAUER, M ^{[1
]}

BAUMANN, J ^{[1
]}

TROMMER, WE ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,FACHBEREICH CHEM,POSTFACH 3049,W-6750 KAISERSLAUTERN,GERMANY

来源：

FEBS LETTERS | 1992年 / 313卷 / 03期

关键词：

BOVINE SERUM ALBUMIN; ATP; SPIN-LABELED-ATP; BINDING STUDIES; FATTY ACID; DOXYL-STEARATE;

D O I：

10.1016/0014-5793(92)81211-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific binding of ATP to bovine serum albumin (BSA) is demonstrated employing ATP derivatives spin-labeled at either N6 or C8 of adenine ring or at the ribose moiety. Based on a 1:1 stoichiometry binding constants are in the 50-100 muM range. Binding is largely competitive with ATP or stearic acid. A small fraction of the labeled nucleotides could not be liberated by these ligands. Binding of AMP is in the millimolar range, only.

引用

页码：288 / 290

页数：3

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