OVEREXPRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROTEASE INCREASES INTRACELLULAR CLEAVAGE OF GAG AND REDUCES VIRUS INFECTIVITY

被引：21

作者：

LUUKKONEN, BGM ^{[1
]}

FENYO, EM ^{[1
]}

SCHWARTZ, S ^{[1
]}

机构：

[1] KAROLINSKA INST, CTR MICROBIOL & TUMORBIOL, S-17177 STOCKHOLM, SWEDEN

来源：

VIROLOGY | 1995年 / 206卷 / 02期

关键词：

D O I：

10.1006/viro.1995.1008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here we have investigated if human immunodeficiency virus type 1 (HIV-1) protease expressed in trans can interfere with production of infectious HIV-1 particles. Protease produced from a Tat and Rev inducible expression plasmid specifically cleaved HIV-1 p55Gag in a dose-dependent manner. Coexpression of protease and an infectious HIV-1 proviral clone resulted in increased intracellular cleavage or p55Gag. As a consequence, virus production and Virus infectivity was significantly reduced. These results suggest that overexpression of HIV-1 protease in HIV-1-infected cells is a powerful way to inhibit production of infectious virions. (C) 1995 Academic Press, Inc.

引用

页码：854 / 865

页数：12

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