The temperature range for induction of formic hydrogenlyase in cell suspensions of psychrophilic strain 82 was 0 C to 20 C compared to 15 C to 45 C for mesophilic Escherichia coli and 15 C to 40 C for mesophilic Aerobacter aerogenes. The respective temperatures for maximum enzyme activity were 30 C, 45 C and 40 C, and for formation of the maximum amount of enzyme 15 C, 35 C and 30 C. The temperatures for all three enzyme functions, therefore, were considerably lower for the psychrophile than for the mesophiles. © 1969 Swets & Zeitlinger.