CELLULAR-LEVELS OF THE PROPHAGE-LAMBDA AND 434-REPRESSORS

被引:30
作者
LEVINE, A [1 ]
BAILONE, A [1 ]
DEVORET, R [1 ]
机构
[1] CNRS,RADIOBIOL CELLULAIRE SECT,ENZYMOL LAB,F-91190 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1016/0022-2836(79)90014-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As a prerequisite to a quantitative study of the inactivation of phage repressors in vivo (Bailone et al., 1979), the cellular concentrations of the bacteriophage λ and 434 repressors have been measured in bacteria with varying repressor levels. Using the DNA-binding assay we have determined the conditions for optimal repressor titration. The sensitivity of the λ repressor assay was increased by adding magnesium ions to the binding mixture; this procedure was without effect on the titration of the 434 repressor. The measures of the cellular repressor concentrations varied with the method of cell disruption. The cellular concentration of λ repressor, about 140 active repressor molecules per monolysogen, was relatively constant under specific cultural conditions. The repressor concentration increased with the number of cI gene copies but not in direct proportion. The 434 repressor concentration, hardly detectable in extracts of lysogens carrying an imm434 prophage, was greatly enhanced in bacteria carrying the newly constructed plasmid pGY101, that encodes the 434 cI gene. The cellular repressor level produced by 434 is lower than that produced by λ: this indicates that the maintenance of the prophage state is ensured by a relatively small number of repressor molecules binding tightly to the operator sites. © 1979.
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页码:655 / 661
页数:7
相关论文
共 27 条
  • [1] BACKMAN K, 1977, THESIS HARVARD U
  • [2] ISOLATION OF ULTRA-VIRULENT MUTANTS OF PHAGE LAMBDA
    BAILONE, A
    DEVORET, R
    [J]. VIROLOGY, 1978, 84 (02) : 547 - 550
  • [3] INACTIVATION OF PROPHAGE-LAMBDA REPRESSOR INVIVO
    BAILONE, A
    LEVINE, A
    DEVORET, R
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1979, 131 (03) : 553 - 572
  • [4] ESCHERICHIA-COLI-K12 INF - MUTANT DEFICIENT IN PROPHAGE LAMBDA INDUCTION AND CELL FILAMENTATION
    BAILONE, A
    BLANCO, M
    DEVORET, R
    [J]. MOLECULAR & GENERAL GENETICS, 1975, 136 (04): : 291 - 307
  • [5] PURIFICATION AND PROPERTIES OF COLIPHAGE-21 REPRESSOR
    BALLIVET, M
    REICHARDT, LF
    EISEN, H
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 73 (02): : 601 - 606
  • [6] LAMBDA-PHAGE AND 434-PHAGE REPRESSORS
    CHADWICK, P
    PIRROTTA, V
    STEINBER.R
    HOPKINS, N
    PTASHNE, M
    [J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1970, 35 : 283 - +
  • [7] COLONY BANK CONTAINING SYNTHETIC COL EL HYBRID PLASMIDS REPRESENTATIVE OF ENTIRE ESCHERICHIA-COLI GENOME
    CLARKE, L
    CARBON, J
    [J]. CELL, 1976, 9 (01) : 91 - 99
  • [8] Davidson N., 1971, BACTERIOPHAGE LAMBDA, P45
  • [9] INTEGRATION-NEGATIVE MUTANTS OF BACTERIOPHAGE LAMBDA
    GOTTESMAN, ME
    YARMOLINSKY, MB
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 31 (03) : 487 - +
  • [10] HARM W, 1963, REPAIR LETHAL ULTRAV