ACIDIC PHOSPHOLIPIDS DIRECTLY INHIBIT DNA-BINDING OF MAMMALIAN DNA TOPOISOMERASE-I

被引：31

作者：

TAMURA, H ^{[1
]}

IKEGAMI, Y ^{[1
]}

ONO, K ^{[1
]}

SEKIMIZU, K ^{[1
]}

ANDOH, T ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

Phospholipid; Topoisomerase I;

D O I：

10.1016/0014-5793(90)80658-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is known to accumulate upon inhibition by a specific inhibitor camptothecin, did not occur. The filter binding assay revealed that the DNA binding activity of the enzyme was inhibited by acidic phospholipids. Moreoever, direct binding of phosphatidylglycerol to topoisomerase I was demonstrated. These results indicated that the inhibitory effect of acidic phospholipids on topoisomerase I was due to the loss of the DNA binding of the enzyme as a result of direct interaction between phospholipids and the enzyme. © 1990.

引用

页码：151 / 154

页数：4

共 31 条

[1] CHARACTERIZATION OF A MAMMALIAN MUTANT WITH A CAMPTOTHECIN-RESISTANT DNA TOPOISOMERASE-I [J].

ANDOH, T ;

ISHII, K ;

SUZUKI, Y ;

IKEGAMI, Y ;

KUSUNOKI, Y ;

TAKEMOTO, Y ;

OKADA, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5565-5569

[2]

BULLOCK P, 1985, SCIENCE, V230, P945

[3] PHOSPHORYLATION OF PURIFIED NOVIKOFF HEPATOMA TOPOISOMERASE-I [J].

DURBAN, E ;

MILLS, JS ;

ROLL, D ;

BUSCH, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 111 (03) :897-905

[4]

FERRO AM, 1984, J BIOL CHEM, V259, P547

[5] DROSOPHILA DNA TOPOISOMERASE-I IS ASSOCIATED WITH TRANSCRIPTIONALLY ACTIVE REGIONS OF THE GENOME [J].

FLEISCHMANN, G ;

PFLUGFELDER, G ;

STEINER, EK ;

JAVAHERIAN, K ;

HOWARD, GC ;

WANG, JC ;

ELGIN, SCR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :6958-6962

[6] DECREASE IN THE DENSITY OF DNA TOPOLOGICAL TURNS DURING INVITRO AGING OF SYRIAN-HAMSTER CELLS [J].

GLASER, VM ;

LUCHNIK, AN .

EXPERIMENTAL CELL RESEARCH, 1982, 139 (02) :249-255

[7] INVITRO TRANSCRIPTION OF EUKARYOTIC GENES IS AFFECTED DIFFERENTLY BY THE DEGREE OF DNA SUPERCOILING [J].

HIROSE, S ;

SUZUKI, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :718-722

[8]

HSIANG YH, 1985, J BIOL CHEM, V260, P4873

[9] INHIBITION OF RETROVIRUS DNA SUPERCOILING IN INTERFERON-TREATED CELLS [J].

HULEIHEL, M ;

ABOUD, M .

JOURNAL OF VIROLOGY, 1983, 48 (01) :120-126

[10]

ISHII K, 1983, J BIOL CHEM, V258, P2728

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