IMMUNOASSAY BY DIFFERENTIAL PULSE POLAROGRAPHY

被引：62

作者：

HEINEMAN, WR

ANDERSON, CW

HALSALL, HB

机构：

[1] Department of Chemistry, University of Cincinnati, Cincinnati

[2] Department of Chemistry, Duke University, Durham

来源：

SCIENCE | 1979年 / 204卷 / 4395期

关键词：

D O I：

10.1126/science.441741

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique. Copyright © 1979 AAAS.

引用

页码：865 / 866

页数：2

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