IMMOBILIZATION OF ALPHA-CHYMOTRYPSIN ONTO HYDROLYZED POLY(ETHYLENE)-G-CO-HYDROXYETHYL METHACRYLATE

被引：13

作者：

DASILVA, MA

GIL, MH

GUIOMAR, AJ

MARTINS, C

GUTHRIE, JT

机构：

[1] UNIV COIMBRA,DEPT CHEM,P-3049 COIMBRA,PORTUGAL

[2] UNIV LEEDS,DEPT COLOUR CHEM & DYEING,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 1990年 / 41卷 / 7-8期

关键词：

D O I：

10.1002/app.1990.070410722

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

α‐Chymotrypsin has been immobilized onto partially hydrolyzed poly(ethylene)‐g‐co‐hydroxyethyl methacrylate (120% graft), using 1‐cyclohexyl‐3‐(2‐morpholinoethyl)‐carbodiimide‐p‐toluene‐sulphonate (CMC) as the activating agent. The influence of the enzyme concentration, the carbodiimide concentration, and the coupling medium on the immobilization reaction was studied. A system with 160 mg of coupled enzyme per gram copolymer, providing 126 mg of active enzyme per gram of copolymer was obtained. The KM, Vmax, and the optimum temperature profile and optimum pH value for the free and immobilized α‐chymotrypsin were determined. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：1629 / 1639

页数：11

共 24 条

[1]

AXEN R, 1971, EUR J BIOCHEM, V18, P351

[2]

BANKS TE, 1969, J BIOL CHEM, V244, P6323

[3] FOOD ADDITIVES - PRODUCTION AND USE OF MICROBIAL ENZYMES FOR FOOD PROCESSING [J].