ISOLATION OF MONONUCLEAR CYTOTOXIC-CELLS FROM CATTLE VACCINATED AGAINST FOOT-AND-MOUTH-DISEASE

被引:18
作者
AMADORI, M [1 ]
ARCHETTI, IL [1 ]
VERARDI, R [1 ]
BERNERI, C [1 ]
机构
[1] SPEDALI CIVIL BRESCIA, BIOTECHNOL LAB, BRESCIA, ITALY
关键词
D O I
10.1007/BF01317191
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mononuclear cells from peripheral blood leucocytes of foot-and-mouth disease (FMD) vaccinated cattle underwent blast transformation after in vitro culture with purified, inactivated, 146 S FMD virus antigen. From the prolonged culture of blast cells in medium with Interleukin-2 (5-10 U/ml), CD 45-positive effector cells were derived, which showed a potent, non MHC-restricted activity against virus-infected cells, the extent of which was inversely correlated with multiplicity of infection (MOI). Extensive characterization of effector cells by means of monoclonal antibodies (MAbs) in flow cytometry, lysis of various cell populations with MAbs and complement, Fc receptor analysis and Cr-51 release assays indicated that the isolated cells share some phenotypic and functional features of the human and murine Large Granular Lymphocyte/Natural Killer (N.K.) lineage.
引用
收藏
页码:293 / 306
页数:14
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