CATABOLISM OF HYALURONAN IN RABBIT SKIN TAKES PLACE LOCALLY, IN LYMPH-NODES AND LIVER

被引:82
作者
LAURENT, UBG
DAHL, LB
REED, RK
机构
[1] UNIV UPPSALA, DEPT OPHTHALMOL, S-75105 UPPSALA, SWEDEN
[2] UNIV TROMSO, DEPT PEDIAT, N-9001 TROMSO, NORWAY
[3] UNIV BERGEN, DEPT PHYSIOL, N-5014 BERGEN, NORWAY
关键词
D O I
10.1113/expphysiol.1991.sp003536
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The catabolism of hyaluronan has been studied by injecting hyaluronan, labelled with I-125-tyramine cellobiose (I-125-TC), subcutaneously into the hindpaw of rabbits. Following endocytosis, I-125-TC remains in the cells at the site of uptake, allowing localization of the site of catabolism. At 6 h after subcutaneous injection, 65% of the injected radioactivity was recovered. The skin at the injection site contained 47%, the popliteal gland at the side of injection 10%, and the liver 8% of the injected dose. At 48 h the three organs contained 40% of the injected dose with 17% in the skin, 10% in the lymph node and 13% in the liver. The decline in recovery could be accounted for by urinary excretion of the tracer, implying that some tracer had been released from the cells after endocytosis. Chromatography revealed that over 85% of I-125-TC-hyaluronan in the lymph nodes and liver was of low molecular mass throughout the experiment. In skin, 4% of the injected tracer was recovered with low molecular mass at 6 h, increasing to 12% of injected dose at 24 and 48 h. Thus, a minimum of 12% of the injected tracer was catabolized per 24 h at the skin injection site. If cells in skin are responsible for the subsequent release of tracer, as seen from the decrease in recovery of the injected dose, another 10-15% of the tracer could have been catabolized locally in the skin per day. The major part of the hyaluronan injected in the skin was, however, catabolized by lymphatic removal and subsequent degradation in local lymph nodes and liver.
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页码:695 / 703
页数:9
相关论文
共 24 条
[1]   ABSENCE OF HYALURONIDASE IN CULTURED HUMAN SKIN FIBROBLASTS [J].
ARBOGAST, B ;
HOPWOOD, JJ ;
DORFMAN, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (01) :376-382
[2]  
BOLLET AJ, 1963, J BIOL CHEM, V238, P3522
[3]   HYALURONIDASE OF RAT SKIN [J].
CASHMAN, DC ;
LARYEA, JU ;
WEISSMANN, B .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1969, 135 (1-2) :387-+
[4]   ON PERICELLULAR ZONE OF SOME MAMMALIAN CELLS IN VITRO [J].
CLARRIS, BJ ;
FRASER, JRE .
EXPERIMENTAL CELL RESEARCH, 1968, 49 (01) :181-+
[5]   PHYSIOLOGICAL FUNCTION OF CONNECTIVE-TISSUE POLYSACCHARIDES [J].
COMPER, WD ;
LAURENT, TC .
PHYSIOLOGICAL REVIEWS, 1978, 58 (01) :255-315
[6]   PREPARATION OF BIOLOGICALLY INTACT RADIOIODINATED HYALURONAN OF HIGH SPECIFIC RADIOACTIVITY - COUPLING OF I-125 TYRAMINE-CELLOBIOSE TO AMINO-GROUPS AFTER PARTIAL N-DEACETYLATION [J].
DAHL, LB ;
LAURENT, TC ;
SMEDSROD, B .
ANALYTICAL BIOCHEMISTRY, 1988, 175 (02) :397-407
[7]   CONCENTRATION OF SODIUM HYALURONATE IN SERUM [J].
ENGSTROMLAURENT, A ;
LAURENT, UBG ;
LILJA, K ;
LAURENT, TC .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1985, 45 (06) :497-504
[8]   UPTAKE AND DEGRADATION OF HYALURONAN IN LYMPHATIC TISSUE [J].
FRASER, JRE ;
KIMPTON, WG ;
LAURENT, TC ;
CAHILL, RNP ;
VAKAKIS, N .
BIOCHEMICAL JOURNAL, 1988, 256 (01) :153-158
[9]  
FRASER JRE, 1989, CIBA F SYMP, V143, P41
[10]   PLASMA-CLEARANCE, TISSUE DISTRIBUTION AND METABOLISM OF HYALURONIC-ACID INJECTED INTRAVENOUSLY IN THE RABBIT [J].
FRASER, JRE ;
LAURENT, TC ;
PERTOFT, H ;
BAXTER, E .
BIOCHEMICAL JOURNAL, 1981, 200 (02) :415-424