IN-VITRO CORM FORMATION AND FIELD-EVALUATION OF CORM-DERIVED PLANTS OF GLADIOLUS

被引：27

作者：

DANTU, PK ^{[1
]}

BHOJWANI, SS ^{[1
]}

机构：

[1] UNIV DELHI,DEPT BOT,DELHI 110007,INDIA

来源：

SCIENTIA HORTICULTURAE | 1995年 / 61卷 / 1-2期

关键词：

GLADIOLUS; IN-VITRO CORM; MICROPROPAGATION; TISSUE CULTURE;

D O I：

10.1016/0304-4238(94)00722-R

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Shoot cultures of Gladiolus cultivar 'Friendship', initiated from axillary buds, exhibited best elongation on liquid Murashige and Skoog's medium (MS) containing 0.5 mg l-1 BAP, in light. On liquid MS medium containing 6% sucrose, in light, 96% shoots formed corms measuring 19.6 mm across and with a fresh weight of 2767 mg. Except for their small size, the in vitro formed corms were comparable with those formed in vivo. Cytokinins (6-benzylaminopurine, kinetin), abscisic acid, CCC, gibberellic acid or activated charcoal either inhibited or had no effect on corm formation. The in vitro formed corms of cultivars 'Friendship', 'Gold Finch's' and 'Her Majesty', treated at 5-degrees-C for 8 weeks showed 100% germination in the field. The micropropagated plants were morphologically and cytologically comparable to the control plants raised from in vivo formed corms.

引用

页码：115 / 129

页数：15

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