Generation of polymerase chain reaction-specific probes for library screening using single degenerate primers

被引：2

作者：

Hommes, NG ^{[1
]}

Arp, DJ ^{[1
]}

SayavedraSoto, LA ^{[1
]}

机构：

[1] OREGON STATE UNIV, LAB NITROGEN FIXAT RES, CORVALLIS, OR 97331 USA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1995年 / 12卷 / 01期

关键词：

single primer PCR; hydroxylamine oxidoreductase; Nitrosomonas europaea;

D O I：

10.1016/1050-3862(95)00101-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Degenerate oligonucleotide primers were made to peptide sequences from hydroxylamine oxidoreductase (HAO) from Nitrosomonas europaea. The primers were used singly in PCR reactions to amplify portions of the gene for HAO from genomic DNA. Southern hybridizations using fragments amplified with each primer showed that they labeled the same genomic DNA fragments. The PCR-amplified fragments were successfully used to screen a gene library for clones containing the HAO gene. The method of isolating genes by PCR with single primers has general utility.

引用

页码：53 / 56

页数：4

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