OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN

被引：52

作者：

MILSTEIN, JM

WHITE, JG

SWAIMAN, KF

机构：

[1] Departments of Neurology and Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota

来源：

JOURNAL OF NEUROCHEMISTRY | 1968年 / 15卷 / 05期

关键词：

D O I：

10.1111/j.1471-4159.1968.tb11627.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation. Copyright © 1968, Wiley Blackwell. All rights reserved

引用

页码：411 / &

共 10 条

[1]

CHANCE B, 1956, ADV ENZYMOL REL S BI, V17, P65

[2] METABOLISM OF RAT BRAIN MITOCHONDRIA DURING POSTNATAL DEVELOPMENT [J].