PROPERTIES OF CHLOROPHYLL-LECITHIN VESICLES - ULTRA-CENTRIFUGATION, ABSORBANCE, EMISSION AND PHOTOBLEACHING

被引:28
作者
DIJKMANS, H
LEBLANC, RM
COGNIAUX, F
AGHION, J
机构
[1] UNIV LIEGE, DEPT BOT, B-4000 LIEGE, BELGIUM
[2] UNIV QUEBEC, DEPT CHIM BIOL, RECH BIOPHYS GRP, TROIS RIVIERES GA9 5H7, TROIS RIVIERES, CANADA
关键词
D O I
10.1111/j.1751-1097.1979.tb07060.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Egg lecithin vesicles (liposomes) containing up to one Chl [chlorophyll] a per 11 lecithin molecules were prepared without sonication. Their absorbance and emission properties indicate that Chl is dispersed in the lecithin, that the vesicles are dispersed in the medium and that there is a strong quenching of fluorescence. Their photobleaching and chemical bleaching show that there is a negligible amount of colloidal Chl present. In vesicles with a Chl:lecthin molar ratio higher than 0.05, there are probably some small aggregates of Chl a in the bilayer. The ultracentrifugation of such vesicles shows that their sedimentation constant increases, though not regularly, with their relative Chl content.
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页码:367 / 372
页数:6
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