THE EFFECT OF IRON AND IRON CHELATORS ON THE INVITRO BLOCK TO DEVELOPMENT OF THE MOUSE PREIMPLANTATION EMBRYO - BAT6 A NEW MEDIUM FOR IMPROVED CULTURE OF MOUSE EMBRYOS INVITRO

被引：123

作者：

NASRESFAHANI, M

JOHNSON, MH

AITKEN, RJ

机构：

[1] MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh EH3 9EW

[2] Department of Anatomy, Cambridge CB2 3DY, Downing Street

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 08期

基金：

英国医学研究理事会;

关键词：

IRON; TRANSFERRIN; INVITRO BLOCK; MOUSE EMBRYO;

D O I：

10.1093/oxfordjournals.humrep.a137235

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The effect of iron and iron chelators on the development of the mouse embryo in vitro from the 1-cell stage to the blastocyst has been investigated. An adverse effect of iron was found. The high affinity iron chelator, desferal, also blocked development, whilst transferrin (whether as apoprotein or saturated with iron), DETAPAC and EDTA promoted development. The addition of transferrin permitted development to the blastocyst stage of embryos from strains normally exhibiting the 2-cell block. Under such circumstances both the rate of embryonic development and the proportion of embryos reaching the blastocyst stage approached levels found in vivo. Based on these results, a new medium, BAT6, is described for the optimal in-vitro culture of mouse embryos.

引用

页码：997 / 1003

页数：7

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