DETERMINATION OF 5-DIMETHYLAMINONAPHTHALENE-1-SULFONYL DERIVATIVES OF URINARY POLYAMINES BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：38

作者：

BROWN, ND

SWEET, RB

KINTZIOS, JA

COX, HD

DOCTOR, BP

机构：

[1] Division of Biochemistry, Walter Reed Army Institute of Research, Washington

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 164卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)81569-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and specific method for the determination of diamines and polyamines by ion-pair high-performance liquid chromatography is described. The 5-dimethylaminonaphthalene-1-sulfonyl derivatives of putrescine, 1,6-diaminohexane, spermidine and spermine are separated on a μBondapak C15 reversed-phase column with 1-heptanesulfonic acid and acetonitrile as the mobile phase. All compounds are eluted within 30 min using a programmed solvent gradient system. The method has a lower detection limit of 1 pmole on column. Because of the simplicity of the method, its application provides a better means for closely monitoring patients undergoing treatment for various types of genito-urinary neoplastic diseases. © 1979.

引用

页码：35 / 40

页数：6

共 9 条

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