MOLECULAR-IDENTIFICATION OF RECEPTOR FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE

被引：77

作者：

OHTAKI, T

WATANABE, T

ISHIBASHI, Y

KITADA, C

TSUDA, M

GOTTSCHALL, PE

ARIMURA, A

FUJINO, M

机构：

[1] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037

[2] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70112

[3] TULANE UNIV,SCH MED,DEPT ANAT,NEW ORLEANS,LA 70112

[4] TULANE UNIV,SCH MED,DEPT PHYSIOL,NEW ORLEANS,LA 70112

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)91222-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide with 38 (PACAP38) or 27 (PACAP27) amino acid residues, structurally related to vasoactive intestinal peptide (VIP). Bovine brain membrane has a PACAP specific receptor interacting with both PACAP27 and PACAP38. Affinity-labeling of the receptor with [125I]PACAP27 identified a dominant band of Mr = 60 k. The labeling density of the 60 k band decreased in the presence of unlabeled PACAP27 or PACAP38, whereas the 60 k band remained in the presence of unlabeled VIP. Binding of [125I]PACAP27 to the membrane decreased in the presence of GTP and the labeling density of the 60 k band decreased concomitantly. The results indicate that bovine brain has a specific PACAP receptor, whose apparent molecular weight is 57 k (substracting the molecular weight of [125I]PACAP27 from 60k). © 1990.

引用

页码：838 / 844

页数：7

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