A GEL-ELECTROPHORESIS ASSAY FOR PHYTOCHELATINS

被引：14

作者：

ABRAHAMSON, SL ^{[1
]}

SPEISER, DM ^{[1
]}

OW, DW ^{[1
]}

机构：

[1] USDA ARS,CTR PLANT GENE EXPRESS,ALBANY,CA 94710

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 200卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90459-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phytochelatins are metal-binding peptides produced by higher plants and some fungi in response to heavy metal exposure. Established methods for analyzing cell-free extracts for the presence of phytochelatins include gel-filtration chromatography and HPLC. We have developed a nondenaturing polyacrylamide gel electrophoresis assay for phytochelatins that combines a small sample size with detection via metal binding. This assay can be used for the measurement of the relative affinity of phytochelatins for a variety of metal and semimetal ions. © 1992.

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页码：239 / 243

页数：5

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