AUTOMATION OF THE BATCH METHOD FOR REACTION KINETIC-STUDIES USING FLOW-INJECTION ANALYSIS - KINETIC-STUDY OF HYDROLYSIS OF N4-ACETYLSULFANILAMIDE, ACETYLSALICYLIC-ACID AND PHENYL PHOSPHATE

被引：4

作者：

KOUPPARIS, MA

ANAGNOSTOPOULOU, PI

GEORGIOU, CA

HADJIIOANNOU, TP

机构：

[1] Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis

来源：

ANALYTICAL LETTERS | 1992年 / 25卷 / 12期

关键词：

AUTOMATED BATCH KINETIC METHOD; FLOW-INJECTION ANALYSIS; N4-ACETYLSULFANILAMIDE HYDROLYSIS; ACETYLSALICYLIC ACID HYDROLYSIS; PHENYL PHOSPHATE ENZYMATIC HYDROLYSIS; ALKALINE PHOSPHATASE;

D O I：

10.1080/00032719208017964

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The automation of the discontinuous (batch) method for kinetic studies using flow-injection analysis (FIA) is described. Aliquots of the reaction mixture are automatically injected in an appropriate manifold and the kinetic profile of the reaction is obtained as a series of absorbance peaks. Observed reaction rate constants are calculated using the Guggenheim and non-linear fitting approaches. The new method is evaluated in the kinetic study of the acid hydrolysis of N4-acetylsulfanilamide by colorimetric monitoring of sulfanilamide, the alkaline hydrolysis of acetylsalicylic acid (aspirin) by colorimetric monitoring of salicylate, and the enzymic hydrolysis of phenyl phosphate with alkaline phosphatase by colorimetric monitoring of phosphate. The automated flow-injection batch method can be used in kinetic studies of reactions with t1/2 greater than 200 s.

引用

页码：2305 / 2327

页数：23

共 36 条

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