SEPARATION OF FLAVONOIDS IN CRUDE EXTRACT FROM SEA BUCKTHORN BY COUNTERCURRENT CHROMATOGRAPHY WITH 2 TYPES OF COIL PLANET CENTRIFUGE

Flavonoid constituents in a crude ethanol extract from dried fruits of sea buckthorn (Hippophae rhamnoides) were subjected to countercurrent chromatography with a two-phase solvent system. Separations were performed with two different types of the coil planet centrifuge: one is called the multilayer coil planet centrifuge and the other, the horizontal flow-through coil planet centrifuge. Although the horizontal flow-through coil planet centrifuge produced efficient peak resolution for five flavonoid components, the multilayer coil planet centrifuge yielded much superior results in terms of partition efficiency, separation time and sample loading capacity.