APHIDICOLIN RESISTANT MUTANT OF WHICH DNA POLYMERASE-ALPHA IS INDUCED BY THIS DRUG

被引：24

作者：

NISHIMURA, M

YASUDA, H

IKEGAMI, S

OHASHI, M

YAMADA, M

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,TOKYO 113,JAPAN

[2] TOKYO METROPOLITAN INST GERONTOL,DEPT BIOCHEM,ITABASHI KU,TOKYO 173,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91970-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutants resistant to aphidicolin, a specific inhibitor of DNA polymerase α of eukaryotic cells, were selected from cultured FM3A cells, derived from mouse mammary carcinoma. One of them, designated as Aph 212, grew in the presence of 1 μg/ml of the drug, which did not permit wild type cells to grow. The resistance of Aph 212 cells to aphidicolin seems to be due to the increment of the activity of DNA polymerase α when Aph 212 cells were cultivated in the presence of the drug. © 1979.

引用

收藏

页码：939 / 945

页数：7

相关论文

共 15 条

[1] ANTIVIRAL EFFECTS OF APHIDICOLIN, A NEW ANTIBIOTIC PRODUCED BY CEPHALOSPORIUM-APHIDICOLA [J].

BUCKNALL, RA ;

MOORES, H ;

SIMMS, R ;

HESP, B .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 4 (03) :294-298

[2] MAMMALIAN-CELLS WITH ALTERED FORMS OF RNA-POLYMERASE II [J].

CHAN, VL ;

SIMINOVITCH, L ;

WHITMORE, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (11) :3119-+

[3] AUTOGENOUS REGULATION OF GENE-EXPRESSION [J].

GOLDBERGER, RF .

SCIENCE, 1974, 183 (4127) :810-816

[4] APHIDICOLIN DOES INHIBIT REPAIR REPLICATION IN HELA-CELLS [J].

HANAOKA, F ;

KATO, H ;

IKEGAMI, S ;

OHASHI, M ;

YAMADA, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 87 (02) :575-580

[5] APHIDICOLIN PREVENTS MITOTIC CELL-DIVISION BY INTERFERING WITH ACTIVITY OF DNA POLYMERASE-ALPHA [J].

IKEGAMI, S ;

TAGUCHI, T ;

OHASHI, M .

NATURE, 1978, 275 (5679) :458-460

[6] RAPID AND CONVENIENT PROCEDURE FOR THE DETECTION OF INHIBITORS OF DNA-SYNTHESIS USING STARFISH OOCYTES AND SEA-URCHIN EMBRYOS [J].

IKEGAMI, S ;

KAWADA, K ;

KIMURA, Y ;

SUZUKI, A .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1979, 43 (01) :161-166

[7]

KNOF KW, 1976, BIOCHEMISTRY, V15, P4540

[8] HYBRIDIZATION OF HAMSTER CELLS WITH HIGH AND LOW FOLATE REDUCTASE ACTIVITY [J].

LITTLEFIELD, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 62 (01) :88-+

[9] MAMMALIAN-CELL MUTANT WITH TEMPERATURE-SENSITIVE THYMIDINE KINASE [J].

NAKANO, MM ;

SEKIGUCHI, T ;

YAMADA, M .

SOMATIC CELL GENETICS, 1978, 4 (02) :169-178

[10] ESTABLISHMENT OF CELL LINES IN VITRO FROM A MAMMARY ASCITES TUMOR OF MOUSE AND BIOLOGICAL PROPERTIES OF ESTABLISHED LINES IN A SERUM CONTAINING MEDIUM [J].

NAKANO, N .

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 1966, 88 (01) :69-84