FACTORS REQUIRED FOR DIFFERENTIATION OF CHICK PRECARDIAC MESODERM CULTURED INVITRO

被引:15
作者
YAMAZAKI, Y
HIRAKOW, R
机构
[1] Department of Anatomy, Saitama Medical School, Saitama 350-04, Morohongo, Moroyama-cho, Iruma-gun
来源
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1991年 / 67卷 / 09期
关键词
PRECARDIAC MESODERM; MYOCARDIUM; DIFFERENTIATION INVITRO; CHICK EMBRYO; INSULIN;
D O I
10.2183/pjab.67.165
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Precardiac mesoderm (PCM) of chick embryo at stage 5 was excised with or without endoderm and ectoderm and cultured in vitro using Eagle's minimum essential medium (MEM) as a basic medium. PCM placed alone in MEM degenerated. When PCM was associated with endoderm, it produced beating tissue in MEM, while it did not show pulsation with ectoderm in the same condition. If MEM was supplemented with 15% horse serum, PCM developed into beating tissue without endoderm. Insulin (25-mu-g/ml) was effective in differentiation of PCM to myocardial cells.
引用
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页码:165 / 169
页数:5
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