IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CIPROFLOXACIN AND ITS METABOLITES IN HUMAN SPECIMENS

被引：39

作者：

MACK, G

机构：

[1] Paediatric Pharmacology Unit, Department of Paediatrics, Westmead Hospital, Westmead

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 582卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(92)80331-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple approach to the quantitation of ciprofloxacin and its three metabolites, M1 (desethylene-ciprofloxacin), M2 (sulfo-ciprofloxacin) and M3 (oxo-ciprofloxacin), in human serum, urine, saliva and sputum is described. This assay allows the parent drug and its metabolites to elute and be resolved in a single chromatogram at 280 nm using a linear gradient. The procedure involved liquid-liquid extraction. Separation was achieved on a C18 reversed-phase column. The limit of detection of ciprofloxacin is 0.05 mug/ml and that of its three metabolites is 0.25 mug/ml. This method is sufficiently sensitive for pharmacokinetic studies.

引用

页码：263 / 267

页数：5

共 8 条

[1] DETERMINATION OF CIPROFLOXACIN AND ITS 7-ETHYLENEDIAMINE METABOLITE IN HUMAN-SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].