INITIAL STEPS IN THE ANAEROBIC DEGRADATION OF 3,4,5-TRIHYDROXYBENZOATE BY EUBACTERIUM-OXIDOREDUCENS - CHARACTERIZATION OF MUTANTS AND ROLE OF 1,2,3,5-TETRAHYDROXYBENZENE

被引:26
作者
HADDOCK, JD [1 ]
FERRY, JG [1 ]
机构
[1] VIRGINIA POLYTECHN INST & STATE UNIV,DEPT ANAEROB MICROBIOL,BLACKSBURG,VA 24061
关键词
D O I
10.1128/JB.175.3.669-673.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chemical mutagenesis and antibiotic enrichment techniques were used to isolate five mutant strains of the obligate anaerobe Eubacterium oxidoreducens that were unable to grow on 3,4,5-trihydroxybenzoate (gallate). Two strains could not transform gallate and showed no detectable gallate decarboxylase activity. Two other strains transformed gallate to pyrogallol and dihydrophloroglucinol but lacked the hydrolase activity responsible for ring cleavage. A fifth strain accumulated pyrogallol, although it contained adequate levels of the enzymes proposed for the complete transformation of gallate to the ring cleavage product. The conversion of pyrogallol to phloroglucinol by cell extract of the wild-type strain was dependent on the addition of 1,2,3,5-tetrahydroxybenzene or dimethyl sulfoxide. This activity was induced by growth on gallate, while the other enzymes involved in the initial reactions of gallate catabolism were constitutively expressed during growth on crotonate. The results confirm the initial steps in the pathway previously proposed for the metabolism of gallate by E. oxidoreducens, except for the conversion of pyrogallol to phloroglucinol.
引用
收藏
页码:669 / 673
页数:5
相关论文
共 24 条
[1]   NEW APPROACH TO CULTIVATION OF METHANOGENIC BACTERIA - 2-MERCAPTOETHANESULFONIC ACID (HS-COM)-DEPENDENT GROWTH OF METHANOBACTERIUM-RUMINANTIUM IN A PRESSURIZED ATMOSPHERE [J].
BALCH, WE ;
WOLFE, RS .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1976, 32 (06) :781-791
[2]   MICROBIAL-METABOLISM OF HOMOCYCLIC AND HETEROCYCLIC AROMATIC-COMPOUNDS UNDER ANAEROBIC CONDITIONS [J].
BERRY, DF ;
FRANCIS, AJ ;
BOLLAG, JM .
MICROBIOLOGICAL REVIEWS, 1987, 51 (01) :43-59
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   PHLOROGLUCINOL PATHWAY IN THE STRICTLY ANAEROBIC PELOBACTER-ACIDIGALLICI - FERMENTATION OF TRIHYDROXYBENZENES TO ACETATE VIA TRIACETIC ACID [J].
BRUNE, A ;
SCHINK, B .
ARCHIVES OF MICROBIOLOGY, 1992, 157 (05) :417-424
[5]   SEQUENTIAL TRANSHYDROXYLATIONS CONVERTING HYDROXYHYDROQUINONE TO PHLOROGLUCINOL IN THE STRICTLY ANAEROBIC, FERMENTATIVE BACTERIUM PELOBACTER-MASSILIENSIS [J].
BRUNE, A ;
SCHNELL, S ;
SCHINK, B .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1861-1868
[6]   PYROGALLOL-TO-PHLOROGLUCINOL CONVERSION AND OTHER HYDROXYL-TRANSFER REACTIONS CATALYZED BY CELL-EXTRACTS OF PELOBACTER-ACIDIGALLICI [J].
BRUNE, A ;
SCHINK, B .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :1070-1076
[7]  
DAGLEY S, 1978, BACTERIA, V6, P305
[8]  
DAGLEY S, 1978, NATURWISSENSCHAFTEN, V65, P89
[9]   BIOCHEMISTRY OF BACTERIAL CATABOLISM OF AROMATIC-COMPOUNDS IN ANAEROBIC ENVIRONMENTS [J].
EVANS, WC .
NATURE, 1977, 270 (5632) :17-22
[10]   ANAEROBIC DEGRADATION OF AROMATIC-COMPOUNDS [J].
EVANS, WC ;
FUCHS, G .
ANNUAL REVIEW OF MICROBIOLOGY, 1988, 42 :289-317