EFFECT OF PROTEOLYTIC-ENZYMES ON THE LYSIS AND GROWTH OF ORAL BACTERIA

被引:22
作者
GRENIER, D
机构
[1] Groupe de Recherche en Ecologie Buccale, Faculté de médecine dentaire, Université Laval, Quebéc, Québec
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 9卷 / 04期
关键词
PROTEOLYTIC ENZYME; PROTEASE; LYSIS; ORAL BACTERIA; ECOLOGY;
D O I
10.1111/j.1399-302X.1994.tb00062.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. The aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. The effect of trypsin, chymotrypsin and proteinase K on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was tested using a disc-plate technique. Overall, gram-positive bacteria appeared to be more resistant to lysis than gram-negative bacteria. The most susceptible bacteria were Actinomyces spp., Eubacterium saburreum, Prevotella intermedia, Capnocytophaga ochracea, Fusobacterium nucleatum, Prevotella loescheii, Treponema denticola and Actinobacillus actinomycetemcomitans. The disc-plate procedure indicated that the growth of Actinomyces spp., E. saburreum, C. ochracea, P. intermedia, P. loescheii, Porphyromonas gingivalis and T. denticola were the most affected, more particularly by chymotrypsin and proteinase K. Interestingly, the growth of F. nucleatum was rather stimulated by proteolytic enzymes. The observations reported in this investigation indicate that specific and general proteolytic activities have the ability to lyse some oral bacterial species and to interfere with their growth. It is suggested that such effects could represent new mechanisms by which the bacterial ecology of subgingival sites may be affected.
引用
收藏
页码:224 / 228
页数:5
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