ESTABLISHMENT AND CHARACTERIZATION OF A PRONEPHRIC STROMAL CELL-LINE (TPS) FROM RAINBOW-TROUT, ONCORHYNCHUS-MYKISS W

被引：18

作者：

DIAGO, MKL ^{[1
]}

LOPEZFIERRO, MP ^{[1
]}

RAZQUIN, B ^{[1
]}

VILLENA, A ^{[1
]}

机构：

[1] UNIV LEON,FAC BIOL,DEPT BIOL CELULAR & ANAT,E-24071 LEON,SPAIN

来源：

FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 06期

关键词：

CELL CULTURE; CELL LINE; PRONEPHROS; STROMAL CELLS; RAINBOW TROUT; FISH;

D O I：

10.1006/fsim.1995.0042

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A new stromal adherent cell line, called trout pronephric stroma (TPS), was initiated from a long-term pronephric culture of an adult rainbow trout, and subcultured 104 times over a period of 4 years. This study describes the culture conditions and characterization by enzyme-cytochemistry, electron microscopy, isoenzyme profile, cytogenetic techniques, and viral susceptibility. The cell types in TPS cultures consisted of fibroblastic, epithelioid and giant cells. The modal chromosome number is 58. The optimum growth temperature of the TPS cells is 18-22 degrees C, with a population doubling time of 27 h. The TPS line is susceptible to viral haemorrhagic septicaemia (VHS) virus and infectious haemopoietic necrosis (IHN) virus. (C) 1995 Academic Press Limited

引用

页码：441 / 457

页数：17

共 43 条

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