SYNTHESIS AND PROPERTIES OF A PHOTOAFFINITY PROBE FOR GLUCAGON RECEPTOR IN HEPATOCYTE PLASMA-MEMBRANES

被引：14

作者：

BREGMAN, MD ^{[1
]}

CHENG, S ^{[1
]}

LEVY, D ^{[1
]}

机构：

[1] UNIV SO CALIF,SCH MED,DEPT BIOCHEM,LOS ANGELES,CA 90033

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 539卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0304-4165(78)90082-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reaction of glucagon with 4-fluoro-3-nitrophenylazide has been shown to afford the photosensitive derivative, Nε{lunate}-4-azido-2-nitrophenyl-glucagon. The structure and properties of this derivative were established by amino acid analysis, absorption and fluorescence spectroscopy, deamination, Edman degradation and photolysis. This photoaffinity derivative of glucagon has been used to label specifically glucagon binding sites on hepatocyte plasma membranes. © 1978.

引用

页码：489 / 495

页数：7

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