THE USE OF URINARY NORMAL-ACETYL-BETA-D-GLUCOSAMINIDASE (NAG) FOR THE DETECTION OF CONTRAST-MEDIA-INDUCED OSMOTIC NEPHROSIS IN RATS

被引：22

作者：

HOFMEISTER, R ^{[1
]}

BHARGAVA, AS ^{[1
]}

GUNZEL, P ^{[1
]}

机构：

[1] SCHERING AG,RES LABS,MULLERSTR 170-178,W-1000 BERLIN 65,GERMANY

来源：

TOXICOLOGY LETTERS | 1990年 / 50卷 / 01期

关键词：

'Osmotic nephrosis'; N-Acetyl-β-d-glucosaminidase (NAG); Non-ionic contrast media; Urinary enzymes; Rat;

D O I：

10.1016/0378-4274(90)90247-J

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Excretion of urinary N-acetyl-β-glucosaminidase (NAG), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and γ-glutamyltransferase (γ-GT) was studied following single intravenous administrations of a non-ionic monomeric contrast medium (iohexol) at doses of 5.0, 7.5, 10.0 and 12.5 g iodine/kg body wt in rats. Measurements of urinary enzymes, serum urea nitrogen and serum creatinine were carried out on the 2nd, 3rd, 4th and 8th days after treatment. Histological examinations of kidneys were performed on days 3 and 8. From 5.0 g iodine/k onwards urinary NAG showed a dose-dependent and significant (multiple comparison, α = 0.05) increase in the first 18-h urine samples after application. A significant increase in urinary LDH could be observed only at the highest dose of 12.5 g iodine/kg. All other biochemical parameters showed no differences when compared to the control group. The dose-dependent increase in lysosomal NAG correlated with the histological findings i.e. there was dose-dependent vacuolization of proximal tubular cells, so-called 'osmotic nephrosis'. © 1990.

引用

页码：9 / 15

页数：7

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