DETECTION OF ANTIGEN IN THE CELOMOCYTES OF THE EARTHWORM, EISENIA-FOETIDA (ANNELIDA)

被引：16

作者：

BILEJ, M ^{[1
]}

ROSSMANN, P ^{[1
]}

VANDENDRIESSCHE, T ^{[1
]}

SCHEERLINCK, JP ^{[1
]}

DEBAETSELIER, P ^{[1
]}

TUCKOVA, L ^{[1
]}

VETVICKA, V ^{[1
]}

REJNEK, J ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS, INST MOLEC BIOL, DEPT GEN BIOL, RHODE ST GENESE, BELGIUM

来源：

IMMUNOLOGY LETTERS | 1991年 / 29卷 / 03期

关键词：

EISENIA-FOETIDA; OLIGOCHAETA; CELOMOCYTE; ANTIGEN RECOGNITION;

D O I：

10.1016/0165-2478(91)90177-C

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Earthworms, Eisenia foetida, are able to respond to antigenic stimulation by the formation of the antigen-binding molecules by coelomocytes - the effector cells of annelids' defence reactions. The ability to react with gold-labelled antigen was detected in agranular coelomocytes by electron microscopy. Furthermore, flow cytometry analysis used for quantitative evaluation of antigen binding showed significant increase in both antigen-binding cells and the amount of antigen bound per cell after stimulation. The antigen binding was inhibited by preincubation of cells with several similar proteins, although the most potent inhibitor was the immunizing antigen.

引用

页码：241 / 246

页数：6

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