PRIMARY STRUCTURE OF THE 2ND LARGEST SUBUNIT OF HUMAN RNA POLYMERASE-II (OR B)

被引：26

作者：

ACKER, J

WINTZERITH, M

VIGNERON, M

KEDINGER, C

机构：

[1] Laboratoire de Génétique Moléculaire, Eucaryotes (CNRS) Unite 184 de Biologie Moleculaire et de Genie Genetique (INSERM), 67085 Strasbourg Cedex

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 226卷 / 04期

关键词：

CDNA; EVOLUTION; HOMOLOGY; SEQUENCE; TRANSCRIPTION;

D O I：

10.1016/0022-2836(92)91071-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA of the second largest subunit of RNA polymerase II (or B) from HeLa cells has been cloned and sequenced. A predicted amino acid sequence of 1174 residues (calculated molecular mass of 133,896 Da) was derived from the longest open reading frame and compared to the sequences of homologous subunits of polymerases of eukaryotic, archaeal and bacterial origin. After optimal alignment, about 16% of the residues were found to be conserved throughout evolution, from human to Escherichia coli. About 2 3 of the overall length of the conserved domains delinated by these residues are clustered within the C-terminal half of the human polypeptide, whereas the remaining is spread over its N-terminal half. The putative functional significance of these conserved domains is discussed. © 1992.

引用

页码：1295 / 1299

页数：5

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