A FAST AND EFFICIENT METHOD FOR SIMULTANEOUS-X AND SIMULTANEOUS-Y INSITU HYBRIDIZATION OF HUMAN BLASTOMERES

被引：79

作者：

MUNNE, S

WEIER, HUG

STEIN, J

GRIFO, J

COHEN, J

机构：

[1] The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York

[2] Division of Molecular Cytometry MCB 230, Department of Laboratory Medicine, University of California, San Francisco, 94143-0808, California

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 01期

关键词：

EMBRYO; PREIMPLANTATION; DIAGNOSIS; X-CHROMOSOME; Y-CHROMOSOME; CHROMOSOME-8;

D O I：

10.1007/BF01204446

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: To use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos. Results: Simultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes. Conclusion: This technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.

引用

页码：82 / 90

页数：9

共 25 条

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