PSEUDOMONAS-AERUGINOSA CORNEAL ULCER ISOLATES DISTINGUISHED USING THE ARBITRARILY PRIMED PCR DNA-FINGERPRINTING METHOD

被引:9
作者
BUKANOV, N
RAVI, VN
MILLER, D
SRIVASTAVA, K
BERG, DE
机构
[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110
[2] WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110
[3] WASHINGTON UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,ST LOUIS,MO 63110
[4] BASCOM PALMER EYE INST,MIAMI,FL 33136
关键词
PSEUDOMONAS AERUGINOSA; CORNEAL ULCERS; KERATITIS; ARBITRARILY PRIMED POLYMERASE CHAIN REACTION (PCR); RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING; HUMAN;
D O I
10.3109/02713689409025132
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Infection of the eye by Pseudomonas aeruginosa can result in corneal inflammation (keratitis) and ulceration, and permanent decrease in vision if not successfully treated. We tested for diversity among P. aeruginosa strains from corneal ulcers by the sensitive and efficient 'RAPD' (for 'random amplified polymorphic DNA') fingerprinting method. This method uses single oligonucleotides of arbitrarily chosen sequence as primers in low-stringency PCR amplification, and results in strain-specific arrays of DNA fragments. Tests of 20 independent P. aeruginosa corneal ulcer isolates yielded 19 different arrays of products with each of three arbitrary primers, indicating that all but two of the strains differed from one another. Additional isolates from three patients (infected eye, contact lens or eye drops) yielded fragment patterns that were identical to those of the original isolate in each case. Thus, our results demonstrate considerable diversity among P. aeruginosa corneal ulcer isolates, and suggest that just one clone may predominate in typical infections.
引用
收藏
页码:783 / 790
页数:8
相关论文
共 21 条
[1]   DNA DIVERSITY AMONG CLINICAL ISOLATES OF HELICOBACTER-PYLORI DETECTED BY PCR-BASED RAPD FINGERPRINTING [J].
AKOPYANZ, N ;
BUKANOV, NO ;
WESTBLOM, TU ;
KRESOVICH, S ;
BERG, DE .
NUCLEIC ACIDS RESEARCH, 1992, 20 (19) :5137-5142
[2]   RESOLUTION OF RECENT EVOLUTIONARY DIVERGENCE AMONG ESCHERICHIA-COLI FROM RELATED LINEAGES - THE APPLICATION OF PULSED FIELD ELECTROPHORESIS TO MOLECULAR EPIDEMIOLOGY [J].
ARBEIT, RD ;
ARTHUR, M ;
DUNN, R ;
KIM, C ;
SELANDER, RK ;
GOLDSTEIN, R .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (02) :230-235
[3]  
BARON EJ, 1990, DIAGNOSTIC MICROBIOL, P39
[4]   DNA-SEQUENCE DIVERGENCE AMONG DERIVATIVES OF ESCHERICHIA-COLI K-12 DETECTED BY ARBITRARY PRIMER PCR (RANDOM AMPLIFIED POLYMORPHIC DNA) FINGERPRINTING [J].
BRIKUN, I ;
SUZIEDELIS, K ;
BERG, DE .
JOURNAL OF BACTERIOLOGY, 1994, 176 (06) :1673-1682
[5]   USE OF PULSED-FIELD GEL-ELECTROPHORESIS FOR EPIDEMIOLOGIC-STUDY OF BORDETELLA-PERTUSSIS IN A WHOOPING-COUGH OUTBREAK [J].
DEMOISSAC, YR ;
RONALD, SL ;
PEPPLER, MS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (02) :398-402
[6]  
ELLSWORTH DL, 1993, BIOTECHNIQUES, V14, P214
[7]   AMPLIFICATION FRAGMENT LENGTH POLYMORPHISM IN BRUCELLA STRAINS BY USE OF POLYMERASE CHAIN-REACTION WITH ARBITRARY PRIMERS [J].
FEKETE, A ;
BANTLE, JA ;
HALLING, SM ;
STICH, RW .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7778-7783
[8]  
GALENTINE PG, 1984, ARCH OPHTHALMOL-CHIC, V102, P891
[9]  
HOLLAND SP, 1993, OPPORTUNIST PATHOGEN, P159
[10]   DIVERSITY AMONG CLINICAL ISOLATES OF HISTOPLASMA-CAPSULATUM DETECTED BY POLYMERASE CHAIN-REACTION WITH ARBITRARY PRIMERS [J].
KERSULYTE, D ;
WOODS, JP ;
KEATH, EJ ;
GOLDMAN, WE ;
BERG, DE .
JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7075-7079