LIQUID-CHROMATOGRAPHIC DETERMINATION OF CARNITINE BY PRECOLUMN DERIVATIZATION WITH PYRENE-1-CARBONYL CYANIDE

被引：9

作者：

KAMATA, K

TAKAHASHI, M

TERASIMA, K

NISHIJIMA, M

机构：

[1] Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo, 169, 24-1 Hyakunincho

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)89058-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of carnitine chloride in pharmaceutical preparations by precolumn derivatization and high-performance liquid chromatography (HPLC). Carnitine chloride is derivatized by reaction with pyrene-1-carbonyl cyanide (PCC) in dimethyl sulphoxide. The PCC derivative of carnitine is then used for quantitative HPLC analysis on a cation-exchange column with fluorescence detection. The calibration graph was linear over a sample concentration range from 2.0 to 20 mu g/ml; the limit of detection was 0.5 mu g/ml under the conditions studied. The proposed method was applied satisfactorily to the determination of carnitine chloride in pharmaceutical preparations.

引用

页码：113 / 118

页数：6

共 17 条

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